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Ramanujam, Bonam
- Evaluation of Beauveria bassiana and Metarhizium anisopliae isolates against Plutella xylostella (L.) under laboratory conditions
Authors
1 ICAR-National Bureau of Agricultural Insect Resources, Post Bag No. 2491, H.A. Farm Post, Bellary Road, Bengaluru - 560024, Karnataka, IN
2 Department of Biotechnology, Center for Post Graduate Studies, Jain University, Jayanagar, Bengaluru - 560011, Karnataka, IN
Source
Journal of Biological Control, Vol 34, No 3 (2020), Pagination: 168-172Abstract
Twenty isolates each of Beauveria bassiana and Metarhizium anisopliae were tested against the second instar larvae of Plutella xylostella through laboratory bioassay using larval dip method at the concentration of 1×107 conidia/ml. Higher larval mortality of 88.85% and 81.44%, were observed with NBAIR Ma-4 and NBAIR Ma-35 isolates of M. anisopliae and 77.36% with NBAIR-Bb-5a isolate of B. bassiana. Further studies on dose and time mortality with four isolates indicated lowest LC50 value of 2.6×104 conidia/ml and LT50 of 86.6 hours with NBAIR Ma-35 isolate. These isolates have to be further tested against P. xylostella in infested fields of cabbage and cauliflower.
Keywords
Beauveria bassiana, Entomopathogenic fungi, Metarhizium anisopliae, Plutella xylostellaReferences
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- Natural occurrence of entomopathogenic fungus, Aschersonia aleyrodis on citrus whitefly, Dialeurodes citri (Ashmead) in Kinnow mandarin in Punjab, India
Authors
1 Department of Fruit Science, Punjab Agricultural University, Ludhiana – 141004, Punjab, IN
2 ICAR-National Bureau of Agricultural Insect Resources (NBAIR), Bengaluru – 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 35, No 3 (2021), Pagination: 181-186Abstract
Surveys were conducted during 2017 and 2018 in the citrus orchards of Punjab, India to record the incidence of different insect pests and their natural enemies. During October-December, Entomopathogenic Fungus (EPF), Aschersonia aleyrodis was found to infect nymphs and pupae of citrus whitefly, Dialeurodes citri on the lower leaf surface of Kinnow from the orchards of Hoshiarpur, Ludhiana, Mansa and Fazilka districts.The fungus was isolated from the infected nymphs and pupae and morphological studies were conducted to confirm the identity of the entomopathogenic fungus. Aschersonia aleyrodis was reported for the first time on D. citri under Punjab conditions and this EPF also confirmed by amplification and sequencing of beta tubulin gene showed 99.40 per cent identity in NCBI, GenBank. Hence further studies on the host range, interaction with other insect pests and parasitoids, survival and longevity should be conducted to explore the potential of this fungus as microbial biocontrol agent for citrus whitefly.